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Static magnetic field can ameliorate detrimental effects of cryopreservation on human spermatozoa

Campo magnético estático puede mejorar los efectos perjudiciales de la criopreservación en espermatozoides humanos

  • Negin Kargar Dahr1
  • Parviz Abdolmaleki2
  • Iman Halvaei1,*,

1Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, 1411713116 Tehran, Iran

2Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-331, 1411713116 Tehran, Iran

DOI: 10.22514/j.androl.2024.012 Vol.22,Issue 2,June 2024 pp.27-34

Submitted: 30 September 2023 Accepted: 29 December 2023

Published: 30 June 2024

*Corresponding Author(s): Iman Halvaei E-mail:


This study aims to improve the freezing-thawing process of human sperm using a static magnetic field. The study included 25 normozoospermic human samples. After an initial evaluation of sperm parameters, samples were prepared by the direct swim-up method. Before freezing, sperm motility, viability, morphology, acrosome reaction and DNA fragmentation rate were assessed. The samples were divided into 4 groups: 0, 1, 5 and 10 mT, and each group was frozen by the rapid freezing method. After thawing, the parameters were re-evaluated and compared between groups. Sperm motility decreased significantly during cryopreservation in all groups. The static magnetic field did not protect against decreased progressive motility after freezing, but the total sperm motility was significantly higher in the 10 mT group compared to the other groups. Sperm viability was higher in the 10 mT group than in the other groups. There was no significant difference in the rate of normal sperm morphology after freezing. The rate of spermatozoa with intact acrosome decreased after freeze-thawing, and the static magnetic field did not protect against the acrosome reaction. The rate of DNA integrity was significantly higher in the 10 mT group compared to the other groups. A static magnetic field with an intensity of 10 mT improved sperm viability and DNA integrity compared to other groups. However, it did not provide significant protection against decreased sperm motility or acrosome reaction.


Este estudio tiene como objetivo mejorar el proceso de congelación-descongelación de los espermatozoides humanos mediante un campo magnético estático. El estudio incluyó 25 muestras humanas normozoospérmicas. Tras una evaluación inicial de los parámetros espermáticos, las muestras se prepararon mediante el método de natación directa. Antes de la congelación, se evaluaron la motilidad, la viabilidad, la morfología, la reacción acrosómica y la tasa de fragmentación del DNA de los espermatozoides. Las muestras se dividieron en 4 grupos: 0, 1, 5 y 10 mT, y cada grupo se congeló mediante el método de congelación rápida. Tras la descongelación, los parámetros se reevaluaron y se compararon entre los grupos. La motilidad de los espermatozoides disminuyó significativamente durante la criopreservación en todos los grupos. El campo magnético estático no protegió contra la disminución progresiva de la motilidad después de la congelación, pero la motilidad total de los espermatozoides fue significativamente mayor en el grupo de 10 mT en comparación con los demás grupos. La viabilidad de los espermatozoides fue mayor en el grupo de 10 mT que en los demás grupos. No hubo diferencias significativas en la tasa de morfología normal de los espermatozoides después de la congelación. La tasa de espermatozoides con acrosoma intacto disminuyó después de la congelación-descongelación, y el campo magnético estático no protegió contra la reacción acrosómica. La tasa de integridad del DNA fue significativamente mayor en el grupo de 10 mT en comparación con los otros grupos. Un campo magnético estático con una intensidad de 10 mT mejoró la viabilidad de los espermatozoides y la integridad del DNA en comparación con otros grupos. Sin embargo, no proporcionó una protección significativa contra la disminución de la motilidad de los espermatozoides o la reacción acrosómica.


Rapid freezing; Sperm cells; Cryoinjury; DNA integrity; Acrosome reaction

Palabras Clave

Congelación rápida; Espermatozoides; Criolesión; Integridad del DNA; Reacción acrosómica

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Negin Kargar Dahr,Parviz Abdolmaleki,Iman Halvaei. Static magnetic field can ameliorate detrimental effects of cryopreservation on human spermatozoaCampo magnético estático puede mejorar los efectos perjudiciales de la criopreservación en espermatozoides humanos. Revista Internacional de Andrología. 2024. 22(2);27-34.


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