An animal model of reperfusion in ischemic corporal tissue and the effect of sugammadex on oxidative injury parameters

Un modelo animal de reperfusión en tejido corporal isquémico y el efecto del sugammadex sobre los parámetros de lesión oxidativa

Background: The aim of this study was to analyze the effect of sugammadex on ischemia-reperfusion injury in corporal tissue. Methods: Eighteen male Wistar Albino strain rats were divided into three groups. Group 1 served as the control group. A priapism model was induced in rats in Group 2 and 3. Rats in Group 3 were additionally administered 4 mg/kg sugammadex intravenously immediately after reperfusion. All rats underwent penectomy for histopathological and biochemical evaluations, and blood samples were collected. Results: In Group 2, total oxidant status (TOS) and malondialdehyde (MDA) levels were significantly higher compared to other groups (p < 0.001 and p = 0.009, respectively). A substantial reduction in both TOS and MDA levels was found in Group 3 (p < 0.001 and p = 0.043, respectively). Group 2 exhibited significantly lower activities of a glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) compared to Group 1 (p = 0.043 and p = 0.024, respectively). In contrast, Group 3 showed significant increases in both antioxidant enzyme activities (p = 0.036 and p = 0.034, respectively). Similarly, the total antioxidant status (TAS) was dramatically higher in Group 3 compared to Group 2 (p < 0.001). Microscopic examinations revealed improvements in vasocongestion, edema, desquamation and inflammation scores in Group 3 compared to Group 2 (p < 0.001). Conclusions: Biochemical and histopathological findings suggest that, sugammadex significantly mitigates oxidative damage parameters in corporal tissue affected by ischemia-reperfusion injury.

Resumen

Antecedentes: El objetivo de este estudio fue analizar el efecto del sugammadex sobre la lesión por isquemia-reperfusión en el tejido corporal. Métodos: Dieciocho ratas macho de la cepa Wistar Albino se dividieron en tres grupos. El grupo 1 sirvió como grupo control. Se indujo un modelo de priapismo en las ratas de los grupos 2 y 3. A las ratas del Grupo 3 se les administró adicionalmente 4 mg/kg de sugammadex por vía intravenosa inmediatamente después de la reperfusión. Todas las ratas se sometieron a penectomía para evaluaciones histopatológicas y bioquímicas, y se recolectaron muestras de sangre. Resultados: En el Grupo 2, el estado oxidante total (TOS) y los niveles de malondialdehído (MDA) fueron significativamente más altos en comparación con otros grupos (p < 0.001 y p = 0.009, respectivamente). Se encontró una reducción sustancial en los niveles de TOS y MDA en el Grupo 3 (p < 0.001 y p = 0.043, respectivamente). El Grupo 2 exhibió actividades significativamente menores de una glutatión peroxidasa (GSH-Px) y superóxido dismutasa (SOD) en comparación con el Grupo 1 (p = 0.043 y p = 0.024, respectivamente). Por el contrario, el Grupo 3 mostró aumentos significativos en ambas actividades enzimáticas antioxidantes (p = 0.036 y p = 0.034, respectivamente). De manera similar, el estado antioxidante total (TAS) fue dramáticamente más alto en el Grupo 3 en comparación con el Grupo 2 (p < 0.001). Los exámenes microscópicos revelaron mejoras en las puntuaciones de vasocongestión, descamación, edema e inflamación en el Grupo 3 en comparación con el Grupo 2 (p < 0.001). Conclusiones: Los hallazgos bioquímicos e histopatológicos sugieren que el sugammadex mitiga significativamente los parámetros de daño oxidativo en el tejido corporal afectado por lesión por isquemia-reperfusión.

Sugammadex; Ischaemia-reperfusion injury; Corporal tissue; Rat

Palabras Clave

Sugammadex; Lesión por isquemia-reperfusión; Tejido corporal; Rata

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